Excessive physiological burden of free radicals leads to oxidative stress which is root cause of various human diseases. Enriching of body systems with antioxidants prevents the onset as well as treats the disease caused due to free radicals and related oxidative stress. Muntingiacalabura Linn. is a folklore medicinal plant used against disease produced due to oxidative stress. Continuous hot percolation process is used with various solvents to prepare the extract from the stem of the plant. The extracts obtained especially petroleum ether & ethanols were subjected to the preliminary phytochemical studies. The phytochemical evaluation shows the presence of flavonoids, phenolic compounds, glycosides, saponins and carbohydrates in ethanolic extracts of the plant. Hence, the ethanolic extract which has the polarity in between was selected for further pharmacological evaluation. The ethanolic extract of stem of Muntingiacalabura Linn. was used for the evaluation of antioxidant activity. Various in-vitro models are used to generate the free radicals and the ability of anti-oxidant to reduce or disproportionate the radical is measured. Among those we have undergone with DPPH free radical scavenging activity. Total DPPH scavenging potential of the extracts at concentration 20µg/ml, 40µg/ml, 60µg/ml, 80µg/ml, and 100µg/ ml were measured and results were depicted in Table No: 3 and Fig.1. Significant DPPH radical scavenging activity was evident at all the tested concentrations of the extract. Thus, from the results of the present study, it was concluded that the ethanolic extract of stem of Muntingiacalabura has very good in-vitro anti-oxidant activity against DPPH method and can be used as a supplementary plant drug for various diseases.