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VOL. 6, ISSUE 6 (2021)
Phytochemical screening, antioxidant activity and cytotoxicity of leaf and bark extract of the Philippine endemic species, Artocarpus blancoi (Elmer) Merr
Authors
Rose Chinly Mae H Ortega, Angeleth U Taotao Grapesy Pink M Alsonado, Mylene M Uy, Olga M Nuneza
Abstract
Bark and leaves of Artocarpus blancoi locally known as Antipolo has been used as folkloric medicine in the Philippines. However, evaluation of the phytochemical constituents and cytotoxicity profile of the bark and leaf extracts of A. blancoi has not been fully elucidated. This study aims to investigate the phytochemical properties, antioxidant activity and cytotoxicity of the bark and leaf extracts of A. blancoi. Plant extracts were prepared through extraction with water, ethanol and ethanol-water. For these extracts at concentrations of 10-, 100-, 500- and 1000 ppm, free radical scavenging activity was determined using 2,2-diphenyl-2-picrylhydrazylhydrate (DPPH) while cytotoxicity was done using Brine Shrimp Lethality Analysis (BSLA) wherein mortality was observed after 6 and 24 hours, respectively. The phytochemical analysis revealed that the bark and leaf extracts of A. blancoi contain alkaloids anthraquinones, cyanogenic glycosides, flavonoids, saponins, steroids and tannins. The ethanol extracts have high mortality of A. salina nauplii (100%) observed at 100ppm to 1000ppm with an LC50 value of <10 after 6 hr and 24 hr exposure while the decoction extracts of both plant parts has an LC50 value of >1000 after 6hr and 24 hr exposure. Bark and leaf extracts of A. blancoi possess strong free radical scavenging activity with LC50 value of 5.2 µg/mL and 5.3 µg/mL, respectively. Presence of phytochemical compounds, strong antioxidant properties and very low lethality in the decoction of the bark and leaf extracts of A. blancoi is a preliminary support for its folkloric medicinal uses.
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Pages:370-376
How to cite this article:
Rose Chinly Mae H Ortega, Angeleth U Taotao Grapesy Pink M Alsonado, Mylene M Uy, Olga M Nuneza "Phytochemical screening, antioxidant activity and cytotoxicity of leaf and bark extract of the Philippine endemic species, <em>Artocarpus blancoi </em>(Elmer) Merr ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 370-376
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