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VOL. 6, ISSUE 6 (2021)
Screening, characterization and molecular identification of metal tolerant microalgae from the natural habitats
Authors
Nagalakshmi B, Uma S, Mahalingam P U
Abstract
The growing industrialization and modern agricultural practices are spread worldwide and leads to adverse impact on the ecosystem. In particular, contamination of heavy metals in the environment becomes a major global concern due to their toxicity and threat to human life and environment. However, in nature, Microalgae have developed an extensive spectrum of mechanisms to cope with heavy metal toxicity. Their wide-spread occurrence along with their ability to grow and concentrate heavy metals, ascertains their suitability in practical applications of waste water bioremediation. Therefore, present study was focused on screening of metal tolerant microalgal strains isolated from natural ecosystem. Four microalgal strains were isolated from fresh water and cultivated in BG 11 medium under controlled condition. All the four strains were identified viz., Lyngbya sp. (Blue green algae), Oscillatoria sp (Blue Green algae), Chlorella sp1 (Green algae) and Chlorella sp2 (Green algae). Followed by all the four microalgal strains were screened for its metal tolerance activity against Zinc, Nickel and Chromium under laboratory conditions. Out of four isolates only two microalgal isolates such as Lyngbya sp and Chlorella sp2 were showed better metal tolerant activity. These two metal tolerant microalgae were authenticated through SEM imaging analysis and molecular sequencing. 16S rRNA molecular sequence data revealed that Lyngbya sp. Showed 95% query similarity with Lyngbyahieronymusii and based on 18S rRNA sequence data Chlorella sp2 showed 97% query similarity with Chlorella sorokiniana.
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Pages:119-126
How to cite this article:
Nagalakshmi B, Uma S, Mahalingam P U "Screening, characterization and molecular identification of metal tolerant microalgae from the natural habitats ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 119-126
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