Tender foliar explants of Jatropha curcus L. (~0.5 - 1.0 cm2) were cultured in half strength MS medium supplemented with various combinations and concentrations of 6-benzylaminopurine (BAP), Kinetin (KIN), Naphthalene acetic acid (NAA) and Thidiazuron (TDZ) along with or without riboflavin. Morphologically distinct semi-friable and nodular type callus formations free of bud formation occurred in media devoid of riboflavin. Callus free adventitious buds with no or less callusing proliferated uniformly on the callus surface in media supplemented with concentrations of riboflavin (0.1, 0.3, 0.5, 1.0 mg.l-1). Maximum of 16.3 ± 1.2 shoot buds and 14.5 ± 0.8 shoots were harvested in 4 weeks in medium supplemented with BAP 2.0 mg.l-1, KIN 0.2 mg.l-1l and NAA 0.1 mg.l-1 together with 0.5 mg.l-1 riboflavin. Callus free bud shoot formation was distinct as 25.3 ± 1.2 buds and 20.7 ± 1.3 shoots were obtained from foliar explants after 6 weeks in media supplemented with this combination. Larger number of buds (38.2 ± 0.8) and shoots (34.1 ± 0.9) were obtained from callus cultured in media enriched with 0.1 mg/l TDZ and 0.5 mg/l riboflavin. The combination of 0.1 mg/l TDZ and 0.5 mg/l riboflavin gave the best regeneration for J. curcas leaf where maximum number of buds (45.6 ± 0.9) and shoots (43.2 ± 0.8) were harvested without intervening callus.