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International Journal of
Botany Studies
ARCHIVES
VOL. 6, ISSUE 6 (2021)
Three simple methodologies for the microanatomical study of dicot leaf samples
Authors
Vigi George M, N Hari
Abstract
The success of the leaf anatomical research depends on how much the tissue or cell characteristics differentiate under the compound microscope. This demands, careful preparation of specimens including sectioning, staining, mounting, and so forth. This is possible only with a feasible and standardised methodology for specimen preparation. In this paper, we recommend three standard and simple methodologies for the preparation of leaf specimens to explore 90% of the anatomical details of a leaf, under basic lab conditions. Leaf anatomical study include-leaf surface, leaf skeleton, transverse section of leaf midrib, lamina, and petiole. All these procedures were standardized for fresh leaf materials from selected dicot taxa.
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Pages:142-144
How to cite this article:
Vigi George M, N Hari "Three simple methodologies for the microanatomical study of dicot leaf samples ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 142-144
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