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VOL. 6, ISSUE 6 (2021)
Phytochemistry, GC-MS, and FTIR analysis of ethanolic extract of alfalfa (Medicago sativa) leaves from Cold Desert Ladakh, India
Authors
Basharat Ali, Yogesh K Rawal, Feroz Ahmad, Ahmad Ali
Abstract
This study was done to examine the phytochemistry of Medicago sativa (Alfalfa) leaves extracted with ethanol. Alfalfa leaves were analysed for their proximate nutritional properties, qualitative, quantitative phytochemistry as well as its antioxidant properties. Identification of various compounds and their functional groups present in the extract was done using GC-MS and FTIR techniques. The results revealed alfalfa leaves have a very good nutritional property with 25% crude protein, 12.91% moisture, 4.35% crude oil and 2.13% crude fiber. Alkaloids, flavonoids, saponins, terpenoids, proteins and phenolic compounds were found in the qualitative phytochemical analysis. The quantitative analysis results showed a good amount of phenolics and flavonoids present in the plant leaves. The free radical scavenging activity by the DPPH method showed a good antioxidant property of the extract. A total of 30 compounds were identified by GC-MS analysis and the FTIR spectrum revealed the presence of amines, alkanes, alkenes, alcohols and sulfoxides.
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Pages:241-246
How to cite this article:
Basharat Ali, Yogesh K Rawal, Feroz Ahmad, Ahmad Ali "Phytochemistry, GC-MS, and FTIR analysis of ethanolic extract of alfalfa (<em>Medicago sativa</em>) leaves from Cold Desert Ladakh, India". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 241-246
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