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VOL. 6, ISSUE 6 (2021)
Assessment of the antioxidant activity and antimicrobial activity of Flemingia vestita root extract
Authors
Bhriganka Bharadwaj, Rahnam B Jyrwa, Rahul Nayak, Surjya Loying, Sangita Boro
Abstract
Many diseases have been treated with herbal remedies throughout the history of mankind. A well-known objective for studying them is the discovery of new bioactive components, in the search for promising drugs. Flemingia vestita is traditionally used to kill intestinal helminths. Many studies have proved the anti helminthic effect of the root extract of the plant in-vitro. The present study was carried out to evaluate the antioxidant activity of Flemingia vestita’s root extract. Extraction was done using Soxhlet apparatus with methanol, ethanol and acetone as solvents for extraction. The antioxidant activity of the extracts was evaluated using DPPH by spectrophotometry. The tuberous root possesses antioxidant properties, scavenging DPPH with IC50 values 14µg/ml, 71µg/ml and 66µg/ml of methanol, ethanol and acetone extract respectively against a standard reference of 5µg/ml of ascorbic acid. TLC evaluation showed presence of bio-molecules such as flavonoids with Rf values 0.55, 0.67 and 0.38 in the methanol extract, ethanol extract and acetone extract respectively. The antimicrobial activity performed by disc diffusion method using methanol, ethanol and acetone extract showed inhibition zone of 26mm, 21mm and 19mm against E. coli and 39mm, 30mm and 16mm against Klebsiella species. The positive control discs (antibiotic discs) mostly showed inhibition zones of 30mm, while that of the negative control (2% DMSO) was negligible. Thus, the study reveals that this plant has the potential to be a better drug compared to the prevailing antibiotics and may be effective to treat various diseases.
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Pages:72-76
How to cite this article:
Bhriganka Bharadwaj, Rahnam B Jyrwa, Rahul Nayak, Surjya Loying, Sangita Boro "Assessment of the antioxidant activity and antimicrobial activity of <em>Flemingia vestita</em> root extract ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 72-76
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