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VOL. 6, ISSUE 6 (2021)
Effect of plant hormones on micropropagation of Plumbago zeylanica (Chitrak)–A versatile medicinal plant
Authors
Smita S Chaudhari
Abstract
Chitrak (Plumbago zeylanica Linn.) is versatile medicinal Plant. Axillary bud cultures of P. zeylanica were used to evaluate effect of different concentrations of BAP; Kinetin; combinations of BAP and IAA; combinations of BAP and IBA in Murashige and Skoog (MS) medium. Axillary buds cultured on these media showed direct shoot regeneration. Bud break was observed 100% in all media used within 6-12 days. Maximum shoot length was observed on MS medium containing 1.5 mg/l Kinetin. Multiple shoots were developed on media containing BAP (0.5 mg/l, 1 mg/l, 2 mg/l, 2.5 mg/l, 3 mg/l); BAP + IAA (1mg/l BAP +1 mg/l IAA, 2mg/l BAP +2 mg/l IAA, 2.5mg/l BAP +2.5 mg/l IAA); BAP + IBA (0.5mg/l BAP +0.5mg/l IBA, 2mg/l BAP +2 mg/l IBA, 2.5mg/l BAP +2.5 mg/l IBA). Callus formation was observed when these in vitro regenerated shoots were cultured on MS medium containing 1mg/l IBA + 0.1 mg /l BAP but when these shoots were cultured on MS medium containing 0.5 mg/l IBA, root formation was observed and small plantlets were formed.
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Pages:816-819
How to cite this article:
Smita S Chaudhari "Effect of plant hormones on micropropagation of <em>Plumbago zeylanica</em> (Chitrak)–A versatile medicinal plant ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 816-819
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