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VOL. 6, ISSUE 6 (2021)
In-vitro glutamine synthetase activity of marine diatom Nitzschia sigma
Authors
G Gunabalan, A Saravanakumar, D Jeyapragash, V Manigandan, N Manivannan
Abstract
Glutamine synthetase, a central assimilatory enzyme for ammonia produced from nitrogen fixation and thus it is extremely important to understand the role of nitrogen in limiting the biomass and growth rate of Nitzschia sigma. Glutamine synthetase activity was determined in-vitro using cells permeabilized by freezing/thawing. Assays for glutamine synthetase activity showed that malachite green assay is approximately 30 times more sensitive than molybdate assay for Pi detection. Cells permeabilized with N-2- hydroxyethylpiperzine-N’-2-ethanesulfonic acid showed higher enzyme activity than the Tris and Imidazole and at the optimum pH 7.9. Moreover, ammonia uptake along with GS activity was measured at 25 ̊ C and showed that nitrate starved cells of N. sigma showed the maximum GS activity (71±5 fmol cell-1 hour-1) along with higher ammonia uptake as compared to nitrate grown cells.
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Pages:804-808
How to cite this article:
G Gunabalan, A Saravanakumar, D Jeyapragash, V Manigandan, N Manivannan "<em>In-vitro </em>glutamine synthetase activity of marine diatom <em>Nitzschia sigma</em> ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 804-808
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