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VOL. 6, ISSUE 6 (2021)
Chromatographic and spectroscopic analysis of traditional medicinal plant Melia azedarach L. stem bark extracts
Authors
Sownthariya C, Shanthi P, Thiripura Sundari U, Priyadharshni K, Anushya S, Jennifer S
Abstract
Melia azedarach L. is a medicinal plant belonging to the family meliaceae. It is an important traditional medicinal plant employed in indigenous system of medicine against several diseases. The current work determines the various bioactive compounds present in stem bark extracts (petroleum ether, acetone, ethanol and methanol) of M. azedarach. Chromatographic (TLC, CC, GC-MS) and spectroscopic (UV-visible and FT-IR) analysis were carried out to determine the bioactive compounds. The findings of thin layer chromatographic study revealed visible spots in varying solvent system with different Rf values. Based on the polarity, Rt and Rf value, methanolic extract was found to be best with three visible spots (0.05, 0.10 and 0.25) among the extracts tested. The column chromatographic analysis showed a maximum of 7 fractions in hexane: methanol (8:2) solvent run ratios with different colours. Two major compounds 9, 12-tetradecadien-1-o1 and 2-Cyclohexyl-4a,7-dimethyl-3,4,4a,5,6,8a-hexahydro-2H-benzoxaine-3-carboitrile have been detected through GC-MS analysis based on retention time. The UV-VIS spectrum profile confirmed the presence of halo compounds, alkyl aryl ether, amine, sulfate, alkanes, alcohols and aliphatic primary amine. The present work authenticates that, the stem bark extract of M. azedarach is found to hold important phytocompounds and serve an ultimate role in further experimentation on its pharmaceutical importance.
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Pages:841-846
How to cite this article:
Sownthariya C, Shanthi P, Thiripura Sundari U, Priyadharshni K, Anushya S, Jennifer S "Chromatographic and spectroscopic analysis of traditional medicinal plant <em>Melia azedarach</em> L. stem bark extracts ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 841-846
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