The cultivated tomato (Solanum lycopersicum L.) is one of the globally important crops for fresh market and food processing industry. It is the second most consumed vegetable after potato and contributes greatly to agro-based industry in the world. Induction of mutation by gamma rays, ethyl methane sulfonate (EMS) and their combined treatments was studied in genotypes of Tomato (Solanum lycopersicum L.). Chemical mutagens like ethyl methansulphonate (EMS) which brings about single nucleotide changes, are widely used for developing mutant populations. The present study the characteristic of gamma rays on seed germination percentage, plant survival percentage, number of leaves, number of branches and plant height of tomato (Solanum lycopersicum L.). Based on LC50 value, five doses 5, 10, 15, 20 and 25KR were selected for laboratory and field study. The Tomato seeds (Solanum lycopersicum) were obtained from the Tamil Nadu Agricultural University (TNAU), Coimbatore, TamilNadu. The genotype seeds were selected to study the effect of physical and chemical mutagens. Combination of gamma radiation and EMS caused more damage followed by EMS treatment and gamma radiation alone in M1 generation. Genotype and mutagen both influenced the production of mutants.