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VOL. 6, ISSUE 6 (2021)
in vitro flowering in somatic nodal bud explants of the cultivated GI tagged Jasmine, (Jasminum sambac Linn.)
Authors
Gokila Lakshmi, G Grace Lydial Pushpalatha, T G Nithya, G C Abraham
Abstract
This report on in vitro flowering of the popular floral crop of Jasminum sambac L. presents interesting insights on a relatively unusual academically interesting spectacle where somatic nodal buds picked from field-grown shoots evinced flowering progressing up to anthesis. Reared on MS Basal Medium (BM) and BM supplemented with certain specific plant growth regulators (PGRs), nodal bud cultures initiated in the months of February and March elicited this response. With segments of floral receptacle and vegetative nodes excised from shoots priming for harvest refrained from displaying this phenomenon on hormone-free medium, while PGRs namely Gibberellic acid (GA3) and Salicylic acid (SA) fortified to the medium helped the elicitation of this response. Results of the study show that certain endogenous stipulations and medium-specific conditions could find a role in triggering the response and influence variations in flower size and quality. Observation made in the different experiments of the study revealed that nodal buds isolated from the flowering twig had the propensity to differentiate a flower more in comparison with the juvenile flower buds induced in situ in floral receptacles. Concomitant development of both vegetative shoot and flower bud has been seen adjacently at opposing axils in a given nodal explant groomed medium with low level of SA implicating that flower production per se could be driven by an inherent stimulus facilitated with SA lest not exerting a determinative influence. Completion of flower development heading to anthesis and the lasting retention of floral organs for over four weeks defying withering is a feature of culture that prompts that the said PGR can be hired judiciously to mediate floral senescence.
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Pages:1248-1256
How to cite this article:
Gokila Lakshmi, G Grace Lydial Pushpalatha, T G Nithya, G C Abraham " <em>in vitro</em> flowering in somatic nodal bud explants of the cultivated GI tagged Jasmine, (<em>Jasminum sambac</em> Linn.) ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 1248-1256
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