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VOL. 6, ISSUE 6 (2021)
Determination of total phenolic and flavonoid contents and antioxidant activity of the stem of Achyranthes aspera L. from garhwal Himalaya
Authors
Seema Devi, Asha
Abstract
Achyranthes aspera L. is an important medicinal plant that grows throughout the tropical and warmer regions of the world. This plant is widely used as anti-microbial, cancer chemo-preventive, nephroprotective, diuretic hepatoprotective, analgesic, anti-inflammatory, hypolipidemic, etc. This work aimed to evaluate phytochemical content, quantification of total phenolic and flavonoid content, and antioxidant activity in the stem of Achyranthes aspera L. in chloroform, methanol, and distilled water extract. The total phenolic content was estimated spectrophotometrically using the Folin Ciocalteu method and the total flavonoid content was measured by aluminum chloride colorimetric assay. Antioxidant activity was evaluated by a 2, 2-diphenyl-1picrylhydrazyl (DPPH) assay. The maximum total phenolic and flavonoid content was found in methanolic extract of the stem - 13.34 mg GAE/g and 49.86mg RE/g respectively. The IC50 value for chloroform, methanol and distilled water were 588.69µg/mL, 26.11µg/mL, and 283.30µg/mL respectively. The highest DPPH radical scavenging activity was observed in methanol extract. This examination ropes a good answer to the utilization of Achyranthes aspera L. from Garhwal Himalaya as a natural antioxidant and in herbal medicine.
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Pages:280-284
How to cite this article:
Seema Devi, Asha "Determination of total phenolic and flavonoid contents and antioxidant activity of the stem of <em>Achyranthes aspera</em> L. from garhwal Himalaya ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 280-284
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