Thousands of different constituents are present in plant extracts. To study the pharmacological efficacy in plant extracts there is a need of separation of the active components present in the plants. The present study was aimed to screen the bioactive compounds of one of the important traditional medicinal herb Acmella calva (DC.) R. K. Jansen (Asteracecae). The ethanolic whole plant extract was analyzed using GC-MS. The compounds found in the extract was related with NIST library. The Gas chromatography-Mass spectrometry study revealed the presence of 25 compounds. The compounds are 2(5h)-Furanone, 3-Hydroxy-4,5-Dimethyl-, Butane, 1,1-Diethoxy-3-Methyl-, 3,3-Diethoxy-2-Butanone, Cyclopentane, 1,1,3-Trimethyl-, 1-Tetradecanol, Dodecanoic Acid, Diethyl Phthalate, 1-Pentadecene, Phenol, 4-Octyl-, Hept-2-Ene, 2,4,4,6-Tetramethyl-, 1-Tridecene, Neophytadiene, 6-Octen-1-Ol, 3,7-Dimethyl-, Propanoate, 2-[Ethyl(Methyl)Amino]-1-Phenyl-1-Propanol, N-Hexadecanoic Acid, Hexadecanoic Acid, Ethyl Ester, Phytol, 9,12-Octadecadienoic Acid (Z,Z), 8,11,14-Eicosatrienoic Acid, (Z,Z,Z), (2e,4e)-N-Isobutyldodeca-2,4-Dienamide, Bicyclo [10.1.0], Tridec-1-Ene, Octadecanoic Acid, N-Isobutyl-(2e,4z,8z,10e)-Dodecatetraenamide, 1,2-Benzenedicarboxylic Acid, Dicyclohexyl Ester, Pentadecanal. The compounds were identified by comparing their retention time and peak area with that of literature data. The compounds identified in the present study are potential phytocompounds, which are used in industries for various purpose like as antioxidant, antifungal agent, pesticide, flavoring agent and to prevent cancer.