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VOL. 6, ISSUE 6 (2021)
Development of In-vitro root and micro tuber for the production of forskolin in Coleus forskohlii
Authors
P Senthilkumar, A Lakshmi Prabha
Abstract
Roots, tuberous roots and micro-tubers were successfully produced from tissue culture derived explants of Coleus forskohlii for forskolin production in in vitro condition. MS medium (Murashige and Skoog 1962) supplemented with IAA, NAA and different concentrations of sucrose were used. Effects of IAA, NAA derivatives were compared for growth and forskolin production in cultured roots on different explants with MS Media under different culture condition. IAA, NAA derivatives induced synthesis of forskolin shows the probability for scaling up of the production of this compound in untransformed root culture. The maximum forskolin content (0.014%) was observed in IAA 1.0+NAA 1.0 mg/l combination after 45 days of in vitro root culture. Micro- tuber and tuberous roots were formed after 60 days at the base of the shoots on the solid MS Medium with different Sucrose concentration. The number of tuberous root was varied from 12 ± 0.20 to 16 ± 0.32/shoots and the number of micro-tubers was varied from 04 ± 0.24 to 11 ± 0.26/shoots. The weight of the tuberous roots and micro-tuber varied from 3.2 ± 0.22 to 4.5 ± 0.34 g/shoots and 1.2 ± 0.22 to 5.1 ± 0.28 g/shoots respectively. Optimum concentrations of Sucrose were most effective in tuberous roots and micro-tuber formation. The maximum micro-tubers (11 ± 0.26/shoots) and forskolin content (0.06%) was observed in IAA 1mg with 70% sucrose concentration. This amount of forskolin content was nearly equal to that of wild plant.
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Pages:1043-1049
How to cite this article:
P Senthilkumar, A Lakshmi Prabha "Development of <em>In-vitro </em>root and micro tuber for the production of forskolin in <em>Coleus forskohlii</em> ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 1043-1049
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