Plumeria rubra L. is an Indian medicinal plant belonging to the Apocynaceae family that was incorporated in the artificial fish diets and evaluated for its Immunogenicity and antigen clearance efficacy in Channa punctatus. Channa punctatus (97 ± 12g) were fed with diets containing Plumeria rubra L. and control groups were fed with a normal diet without Plumeria rubra L. After 4 weeks of continuous feeding, fishes were immunized intraperitoneally with the bovine serum albumin. Channa punctatus were sampled for their serum, four times/week @ regular intervals after immunization; and spleen was sampled from C. punctatus on the second week alone. Antibody response specific to bovine serum albumin (BSA) was determined by using ELISA in C. punctatus; antigen clearance was determined by immuno-electron microscopy in the sampled spleen of C. punctatus. It was found that P. rubra L. significantly (p<0.05;0.00167) enhanced the antigen-specific antibody response in C. punctatus. It was also found that C. punctatus treated with P. rubra L., significantly cleared the injected antigen, i.e., BSA.