Sesame is a most medicinal value rich oil seed crop. Plant growth promoting rhizobacteria was most predominant microbes present in the root region of the soil. Phosphate is the one of the most major nutrient for crop growth and development. In oil seeds phosphorus play an important role in the quality of the oil. Using of phosphate solubilizing bacteria can solubilize the unavailable form of phosphorus in to available form. In the present study focus on isolation, characterization and efficiency of the different phosphobacteria isolates from sesame crop. There are 26 different phosphate solubilizing bacteria can obtain and designated as Sesame Rhizosphere Phosphobacteria (SRP) from different locations of Cuddalore district Tamil Nadu, India. Among the 26 isolates based on the phosphate solubilizing index only five isolates SRP 4, SRP 6, SRP 11, SRP 16 and SRP 21were efficient phosphate solubilizers. So the five isolates was morphologically and biochemically characterized as two different genera Pseudomonas sp., and Bacillus sp., among the plant growth promoting activity of the different phosphobacteria isolates SRP 16 was recorded maximum production of IAA, GA3, cytokinin, EPS and ACC deaminase production. Also, the phosphobactera isolate SRP 16 only can able to grow under 25 to 45 °C temperature at 4 to 9 level of pH. Other isolates were found to grow only at the temperature of 35°C, at 4 to 9 tested pH.