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VOL. 6, ISSUE 6 (2021)
Bioprospecting of Rhynchosia rufescens (Willd.) DC. an underutilized wild vegetable
Authors
Vilas T Patil, Madhuri V Suryawanshi, Varsha D Jadhav, Kailas D Sonawane, Priyanka S Dhotare
Abstract
In the current study immature and mature seeds of Rhynchosia rufescens were used for the analysis of antioxidant, secondary metabolites and antibacterial activities. For enzymatic and non-enzymatic antioxidant analysis there are four different solvents like methanol, ethanol, acetone and aqueous with four different concentrations 1mg, 2mg, 3mg and 4mg was used. A unique standard approach was used to assess the antioxidant potential and secondary metabolites of all levels in terms of total alkaloid, total flavonoid, total phenolic, DPPH free radical scavenging potential, ferric ion reducing antioxidant power, reducing potential, total antioxidant capacity, ABTS free radical scavenging potential, ferrous ion chelating ability. In contrast to the other four solvents, acetone plant extract showed the highest antioxidant and secondary metabolite activities. The activities increase as the sample concentration increases from 1 mg to 4 mg. Two Gram positive bacteria, such as Staphylococcus aurous and Bacillus cereus, and two Gram negative bacteria, such as Proteus vulgaris and Escherichia coli, were utilized in antibacterial research. Using the agar well diffusion method, the inhibition zones were calculated. Plant crude extract was compared to standard antibiotic discs and evaluated using bacterial strains. Bacillus cereus was the most active antibacterial strain among the four.
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Pages:1130-1138
How to cite this article:
Vilas T Patil, Madhuri V Suryawanshi, Varsha D Jadhav, Kailas D Sonawane, Priyanka S Dhotare "Bioprospecting of <em>Rhynchosia rufescens</em> (Willd.) DC. an underutilized wild vegetable ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 1130-1138
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