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VOL. 6, ISSUE 6 (2021)
in vitro regeneration of some diploid and triploid mulberry (Morus spp.) varieties using shoot tips as explant
Authors
M Swetha Priya, P Sujathamma
Abstract
The experiment was conducted to study the of micropropagation efficiency in diploid and triploid mulberry (Morus spp.) varieties i.e S13, RFS135, TR10, Vishala, M5 and G2, using shoot tip as explants. MS medium fortified with different concentrations and combinations of growth hormones were used. Alone BAP and kinetin were used in initiation media. BAP 2.0 mg/l was found to be most effective in inducing sprouting in most of the varieties. Initiated shoots were transferred to the multiplication medium containing BAP (0.5 mg/l-2.5 mg/l) + NAA (0.5 mg/l and 1.0 mg/l) and BAP (0.5 mg/l-2.5 mg/l) + kinetin (0.5 mg/l and 1.0 mg/l). BAP 2.0 mg/l in combination with kinetin 1.0 mg/l was efficient in multiple shoots formation. The micro shootlets were subcultured to obtain sufficient growth of the multipleshoots. The results indicated that explants inoculated in alone BAP has showed high percent of shoot initiation. For multiple shoot formation BAP in combination with kinetin was found to be effective in inducing efficient multiple shoots. Two auxins tested for root initiation, NAA showed best response in Vishala and TR10 varieties and average number of roots/shootlet was found to be maximum in triploid varieties.
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Pages:1153-1159
How to cite this article:
M Swetha Priya, P Sujathamma "<em>in vitro</em> regeneration of some diploid and triploid mulberry (<em>Morus</em> spp.) varieties using shoot tips as explant ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 1153-1159
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