Endophytic bacteria are the plant beneficial bacteria that thrive inside plant tissue and can improve plant growth without causing any negative impact. In the present study endophytic bacteria were isolated from leaf, rachis, rhizome and root of an edible fern Ampelopteris prolifera (Retz.) Copel were screen for Indole Acetic Acid (IAA) production. Three isolates RART4, WARH3 and WART2 were identified as Bacillus megaterium (MZ310576), Lynsinibacillus varians (MZ310567) and Paenibacillus sp. (MZ310568) respectively based on 16s rDNA sequencing were produced significant amount of IAA in the presence of 0.1% tryptophan. We have observed RART4 produced highest amount of IAA among the isolates. The enzymatic activities, sugar fermentation and antimicrobial agent resistance abilities of the isolates were recorded. In vitro treatment of the isolate RART4 for plant growth promotion resulted in substantial increase in root, shoot, primary and secondary leaf length, biomass, chlorophyll, and protein content as compared to the control.