The characterization and evaluation of Ethanol extract of Momordica charantia seed (EEMCS) on blood coagulation, platelet function and antioxidant potential was carried out. Phytochemical screening of EEMCS was positive for Polyphenols, saponins, steroids, terpenoids and glycosides. The characterization of EEMCS by TLC and GC/MS adjudged the presence of secondary metabolites. In specific the GCMS data revealed the presence of 16 compounds along with hydrocarbons and eight aldehyde, three esters, three alcohols, one ketone and one terpenic oxide groups. Interestingly, EEMCS was found to be poor anticoagulant because it did not interfere in plasma coagulation in clotting time from control 146s to 160s at 20μg concentration. Furthermore, the effect of EEMCS on platelet aggregation was analyzed using agonists such as collagen, ADP and epinephrine. Interestingly, EEMCS showed 19%, 91% and 97% platelet aggregation inhibition for said agonists in platelet rich plasma. The EEMCS was also examined for its probable antioxidant potential. Oxidative stress was induced using platelet rich plasma using sodium nitrate. To the surprise, EEMCS exhibited strong antioxidant activity that was compared with positive control vitamin C. In addition, EEMCS normalized the levels of stress markers such as, protein carbonyl content, lipid peroxidation, total thiol content, catalase and super oxide dismutase activity in platelet rich plasma. While, EEMCS devoid of RBC lysis, edema and hemorrhagic properties. Suggesting ethanol extract of Momordica charantia seed extract exhibits poor anticoagulant but strong antiplatelet and antioxidant properties.