ARCHIVES
VOL. 6, ISSUE 6 (2021)
In vitro cell selection of chickpea against culture filtrate of R. bataticola with different concentrations
Authors
G Sujith, Shashi Tiwari, Pragati Misra
Abstract
In a procedure of in-vitro callus multiplication for resistant callus to Rhizoctonia bataticola., it was observed that the fast growth of callus cultures were established only from seeds of chickpea. Which are treated with 0.2% bavistin for 6 min, 0.1% mercury chloride for 2 min and 20% sodium hypochlorite for 5min. Pale, friable white color growth of callus was obtained after 30-35 days on MS basal medium containing 2.0 mg/l NAA and 0.5 mg/l BAP and screened in-vitro for resistance to Millipore-filtered purified culture produced by the fungus R. bataticola for creating resistance to dry root rot disease in chickpea. Calli were challenged by different concentrations of culture filtrate of R. bataticola. A cell survival rate of 6.66% at a 18% selective dose of culture filtrate was achieved in unselected calli while at>18% concentration of culture filtrate, results in the death of 100% calli after 3 weeks. However, calli survived within a range of 30 to 65% when subjected to 10-2% fungal culture filtrate indicating low survival rate at higher concentration and high survival rate at lower concentration.
Download
Pages:1351-1356
How to cite this article:
G Sujith, Shashi Tiwari, Pragati Misra "<em>In vitro</em> cell selection of chickpea against culture filtrate of <em>R. bataticola </em>with different concentrations ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 1351-1356
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.