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VOL. 6, ISSUE 6 (2021)
Effect of various plant growth regulators on in vitro root proliferation studies of Spilanthes acmella L. (Murr.) medicinal plant
Authors
H D Chandore
Abstract
In-vitro propagation studies are effective tool for the conserving rare medicinal plants. Spilanthes acmella Mur., is a well-known medicinal plant which termed as antitoothache plant because of its major medicinal properties. Over exploitation of these plant will leads to use of in-vitro propagation studies in different plant growth regulators. Results revealed that in nodal segment explant the use of IBA hormone (3 mg/l) along with MS media are most effective in earlier root formation (16.50 days) along with 80 % vigorous root formation response. Same concentration gives 25.5 number of roots/per explant and maximum length (13.5 cm) within eight weeks. In auxin and cytokinin combinations studies maximum number of roots (21.2) and root length (11.8 cm) in addition with 80 % vigorous root formation has reported in IBA (1 mg/l) when combined with BAP (3 mg/l). The roots are thicker, longer, fibrous, healthy and vigorously grown in these combinations than any other combination. The hardening responses has recorded with different media combination and coco peat (80 %) + soil (20 %) are found to be the best suitable media for its 100 % survival.
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Pages:1328-1332
How to cite this article:
H D Chandore "Effect of various plant growth regulators on in vitro root proliferation studies of <em>Spilanthes acmella L. (Murr.) </em>medicinal plant ". International Journal of Botany Studies, Vol 6, Issue 6, 2021, Pages 1328-1332
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