Urginea is a bulbous perennial plant found in India, Africa, Mediterranean regions of the world. Urginea is represented in India by about nine species (U. indica, U.coramandaneliana, U.govindappae, U.nagarjunae, U.polyantha, U.raogibikii, U.razzi, U.wightii, and U.polyphylla). We carried out Karyomorphological studies in five different Urginea wightii species collected from Gulbarga, Karnataka, India. Each plant of Urginea species varied in its morphology and ploidy. We noticed that in U. wightii of Gulbarga, there were triploid number of chromosomes which showed variation. Chromosome morphology in each plant is distinct. 'St' type of chromosome was a major component of the karyotype followed by ‘t’ type with asymmetrical karyotype. Polysomaty is responsible for the origin of new genotypes (with different chromosomal configurations) through vegetative propagations. These cytotypes, morphotype and ecotypes can be used as tools to study diversification in Urginea species.