An experiment was conducted at experimental farm of Chandigarh University, Mohali, Punjab, during Rabi season of 2020-2021 to study “Effect of split application of nitrogen and phosphorus on growth and yield of wheat (Triticum aestivum L.).” The treatments consisted of various combination of nitrogen along with phosphorus (T1-Nitrogen 125% + Phosphorus 100%, T2- Nitrogen 125% + Phosphorus 75%, T3- Nitrogen 100% + Phosphorus 100%, T4- Nitrogen 100% + Phosphorus 75%, T5- Nitrogen 75% + Phosphorus 100%, T6- Nitrogen 75% + Phosphorus 75% and control). Experiment was conducted under randomized block design (RBD) with three replications. It was concluded that there is increase in spike length (22.47%), number of grain (10.19%), test weight (11.59%), grain yield (15.15%), straw yield (13.10%) and harvest Index (2.01%) with the combined application of nitrogen and phosphorus in treatment T7 (125 kg N/ha +75 kg P/ha) as compared to control. Overall results indicate that in treatment T7 combination of nitrogen along with phosphorus is effective for better growth and yield of wheat as compared to others treatments.