Synthesis of metal oxide nanoparticles is an expanding area of research due to their potential applications in the development of novel technologies. An ecofriendly based synthesized nanoparticles has become an important branch of nanotechnology. in the present work, we have developed the synthesis of silver Nanoparticles (AgNPs) using aqueous leaf extract of Selaginella tenera act as a reducing agent/ capping agent. Formations of green synthesized silver nanoparticles were characterized by UV- visible spectroscopy (U-Vis), X- ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM) analysis. Further, the green synthesized silver nanoparticlesare stabilized by plant metabolities, and their significant antioxidant activity of 1, 1-diphenyl-2-picrylhydrazyl (DPPH)free radicals were effectively. Furthermore, the green synthesized silver nanoparticles showed significant bactericidal effect against four pathogenic bacterial strains namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcushaemolyticus and Staphylococcus aureus shows zone of inhibition by agar well diffusion method. Finally, the investigation suggests that green synthesized silver nanoparticles arehaving potential applications for various industrial fields and also helpful to the scientific community.