This study aims to investigate the Morpho-phytochemical and antibacterial properties of Withania somnifera, particularly focusing on different soil conditions and climatic factors in Agra influence its immune-boosting capabilities. Morphological studies showed improvements in plant height, flower, fruit, and seed parameters when grown in vermicompost. Notably, the shape and size of the roots were significantly enhanced in the vermicompost mixture. Phytochemical analysis of methanol extracts from the roots of Withania somnifera, cultivated under different soil conditions, confirmed the presence of alkaloids, glycosides, flavonoids, phenols, saponins, steroids, and tannins. Antibacterial testing of methanolic root extracts revealed effectiveness against pathogens such as E. coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis. These findings corroborate the traditional medicinal use of Withania somnifera for combating microbial infections, underscoring the potential of plant-based products as sources of antimicrobial