Tulsi or holy basil (Ocimum sanctum L) is a common plant seen in most of the Indian household. It is treated as a spiritual plant but scientifically it is one of the immediate antibiotics in hand. It is also one of the plants used extensively in Ayurvedic medicines where of all the herbs used, Tulsi is the most important herb in Ayurveda, and modern study is now verifying its health benefits.It has a unique combination of pharmacological activities that can relieve physical, physiological, metabolic, and psychological stress. Tulsi has also been proven to help with metabolic stress by lowering blood sugar, blood pressure, and cholesterol levels, as well as mental stress by improving memory and cognitive performance and acting as an antipanic and anti-depressive agent. Its broad-spectrum antibacterial action, which includes activity against a variety of human and livestock infections, implies that it could be utilized as a hand sanitizer, mouthwash, and water purifier, as well as in animal husbandry, wound repair, food preservation, etc. The study gives an insight about the preferable media with given composition which can be used for the micropropagation of Tulsi.The selected explants of the shoot were inoculated in vitro in the growth media i.e., Murashige and Skoog media with varying concentrations of auxin and cytokinin, (2,4-D, BAP, IBA). The explants showed results after 22 days (about 3 weeks) of inoculation, where callusing was observed in nodes, internodes, and young inflorescence.