Cochlospermum religiosum (Linn.) Alston. (Bixaceae) Traditionally, this plant is used for the treatments of jaundice, coughs, tuberculosis, asthma, inflammation, fever gonorrhea, and diarrhea. The current paper reports on morphology and histology studies of the roots of Cochlospermum religiosum Linn. Some unique and distinct characters have been identified in the root category. Physiochemical parameters and preliminary phytochemical studies of leaf powder were also performed. Also measure the amount of phenolic content, flavonoid content, flavonone determination and evaluate the in vitro antioxidant activity of alcohol and aqueous extract from the leaves of the Cochlospermum religiosum Linn. The total phenolic content was calculated calorimetrically using a folin ciocalteu reagent, and the total flavonoid content was determined by aluminum chloride method, while a modified 2, 4-dinitrophenylhydrazine method was used to determine flavonone. Total phenolic content of hydroalcoholic extract, a fraction of chloroform, a fraction of butanol and an insoluble portion of butanol hydroalcoholic extract of the root (active extract) of C. religiosum was found to be 43.50, 18.66, 26.50 and 13.86 mg / g respectively, the total flavo content for the same extraction was found to be 14.2, 11.2, 18.2 and 3.8 mg / g respectively. The total flavonone content for the same extract was found to be 6.0, 0.6, 5.4 and 1.0 respectively. Antioxidant activity was measured based on DPPH solid extracts test, IC50 hydroalcoholic extract, chloroform fraction, butanol fraction and soluble butanol were found to be 117.9 μg / ml 143.45 μg / ml 124.6 20m ml.