In vitro biomass accumulation and regeneration of potential medicinal plant green chiretta-Andrographis paniculata (Burm.f.) nees
Sumitha Jagadibabu, Saritha Baskar2 Arjun Pandian
The plant Andrographis paniculata (Burm.f.) Nees comes under the Acanthaceae family, its widely used around the worldwide used for cold, diabetes, liver disorders, upper respiratory tract infections, snake bite, dysentery, fever, malaria, etc., An efficient protocol was established for rapid micropropagaion A. paniculata from nodal explants. MS medium with IAA (2 mg-1) showed highest growth in terms of fresh and dry weight was observed (44.5/8.7 mg), BAP (0.5 mg-1) showed (44.7/8.5 mg), NAA (1 mg-l) obtained more yielded the greatest fresh/dry weight 24.8/3.5 mg from the nodal explants, 2,4-D (5 mg-l) observed more yielded of fresh/dry weight 60.5/10.6 mg. The combination of IAA and BAP (2+0.5 mg-l) utmost growth occurred fresh/dry weight (79.7/17.3 mg), numerous shoots (20-80%) on the medium with 2, 4-D+NAA+BAP (5+2+1 mg-l) showed multiple shoot formations. The shootlets has high elongation capacity: rooting from callus was not occurred in the nodal explants but minimum rooting performance in the medium complement with IAA (1 mg-l). Well growned shoots were excised from the medium supplemented with 2, 4-D+NAA+BAP (5+2+0.5 mg-l) and transferred to IAA (1 mg-l). After one week from the subculture, few numbers of roots initiation stared from the cut ends of transferred shoots on the medium with IAA (1 mg-l), concluded that competent protocol has been developed for plant regeneration from the calli derived from nodal explants of green chiretta.