International Journal of Botany Studies

International Journal of Botany Studies


International Journal of Botany Studies
International Journal of Botany Studies
Vol. 7, Issue 1 (2022)

GC-MS studies on variation in the content of major chemical constituents of Artemisia annua L. collected from different geographical regions of India


Alka Dangash, Bhavna Sharma, Shivangi Purohit, Neeta Pandya, Dharamchand Jain

GC-MS analysis of Artemisia annua L. leaves samples were done for the samples collected from different geographical in India: A-1 (Uttar Pradesh), A-2 (Jaora, Madhya Pradesh), A-3 (Uttarakhand), A-4 (Baroda, Gujarat), A-5 (Andhra Pradesh), A-6 (Karakhadi, Gujarat). Only five major components, in each geographical area, have been analyzed for their mass-spectrometric fragmentation pattern for identification of major components present in extract samples. Components detected were identified by comparing mass spectral data of samples with those of NIST and NBS standard database hits provided with GC-MS analysis. All the samples differed in their chemical constituents quantitatively. All the samples showed the presence of Artemisinin and deoxyartemisinin. Despite unanimous presence of Artemisinin and deoxyartemisinin, both the compounds differed quantitatively in every sample. Dihydroartemisinic acid, dihydroartemisinic alcohol, artemisinin and deoxyartemisinin were present in extracts of the samples collected from Uttar Pradesh (A-1), Jaora, Madhya Pradesh (A-2), Uttarakhand (A-3), Baroda, Gujarat (A-4) and Kharkhedi, Gujarat (A-6), in addition extract from Uttar Pradesh (A-1) showed the presence of α-amyrin, while extracts collected from Jaora, Madhya Pradesh (A-2), Uttarakhand (A-3) and Baroda, Gujarat (A-4) showed dihyroartemisinic aldehyde, and extract of sample collected Kharkhedi, Gujarat (A-6) showed dihydroartemisinic acid. Comparatively, samples collected from Andhra Pradesh (A-5) and Kharkhedi, Gujarat (A-6) showed clear deviation in chemical constituents from rest of samples. α-amyrin was present in two samples (A-1 and A-5) while β-amyrin is present only in one sample (A-5) which was collected from Andhra Pradesh. Artemisinin content was highest in sample collected from Uttar Pradesh.
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How to cite this article:
Alka Dangash, Bhavna Sharma, Shivangi Purohit, Neeta Pandya, Dharamchand Jain. GC-MS studies on variation in the content of major chemical constituents of Artemisia annua L. collected from different geographical regions of India. International Journal of Botany Studies, Volume 7, Issue 1, 2022, Pages 281-290
International Journal of Botany Studies International Journal of Botany Studies