Top rot of bajra caused by Fusarium verticillioides is one of the destructive disease wherever bajra is grown. In the present study, antagonistic activity of five Trichoderma spp. was tested in vitro against Fusarium verticillioides sensitive and resistant to Roko (Thiophanate methyl 70% WP) in dual culture technique. As compared to other Trichoderma spp used, Trichoderma harzianum was most effective against F. verticillioides controlling the pathogen above 72.84% and 76.17% in sensitive and resistant isolates respectively. Use of Trichoderma spp. for controlling top rot of bajra is ecofriendly technique that can serve as the good alternative to avoid harmful effect of chemical fungicides.