Silver nitrate: A potent anti-ethylene compound stimulates organogenesis in naga chilli (C. chinense Jacq.)
Geetashree Bora, Pratap Jyoti Handique
Northeast India is considered to be one of the prominent centres of chilli pepper diversity. Naga chilli locally known as Bhot jolokia is extensively cultivated all over northeast India including Assam. This chilli possesses high commercial value owing to pungency factor called capsaicin. Despite its tremendous pharmaceutical properties, the germplasm is at risk owing to natural cross pollination and mutation thereby reducing its capsaicin content. This communication brings highlights a complete standardized in vitro micropropagation protocol in Naga Chilli (Bhot jolokia) from leaf explants under the influence of silver nitrate. In vitro propagation was carried out with leaf explants. MS medium was used as the basal medium supplemented with different combinations of plant growth hormones. Addition of Silver nitrate (AgNO3) to the culture medium along with phytohormones (BAP, GA3, 2,4-D and IBA) greatly improved proliferation in the selected in vitro plantlets. AgNO3 effectively induced prolific regeneration of multiple shoots. In vitro flower and fruit induction processes were found to be more productive in the treatment where AgNO3 was combined with GA3 in the culture medium. In vitro root initiation in Naga chilli was very responsive in MS medium supplemented with IBA Potting material fortified with vermicompost and vermiculite greatly improved the chances of acclimatization of in vitro raised healthy plantlets. Plant survival percentage was 86.65%.