International Journal of Botany Studies

International Journal of Botany Studies


International Journal of Botany Studies
International Journal of Botany Studies
Vol. 7, Issue 1 (2022)

Effect of different plant hormones on in vitro plant regeneration via somatic embryogenesis in Cajanus cajan L. millsp.


Manisha Sharma

In the present study, regeneration of Pigeon pea (Cajanus cajan L. Millsp), belongs to family Fabaceae was achieved via somatic embryogenesis. Mature cotyledons were cultured on MS, B5 and modified MS media (MSB5). The media were supplemented with different concentrations of various auxins, viz, IAA/IBA/NAA/2, 4-D (1.0 to 6.0 mg/L) and cytokinins, viz, BAP/kn (1.0 to 6.0 mg/L) alone as well as in various combinations. Besides auxins and cytokinins, adenine sulphate was also incorporated into the media at various levels (50 to 150 mg/L). Embryogenic callus was induced on modified MS media augmented with 2, 4-D (3.0 mg/l) BAP (5.0 mg/l) and adenine sulphate (100 mg/l). At this particular combination, nodular embryogenic callus was obtained within 2-3 weeks. Further proliferation and maturation of these somatic embryos was achieved on MSB5 medium containing reduced concentrations of hormones, i.e. 2, 4-D (1.5 mg/l), BAP (2.5 mg/l) and adenine sulphate (50 mg/l). In about 20-30 days, the somatic embryos depicted typical stages of embryo development. Germinating embryos showed well developed shoot, but meager root initiation. Therefore, these were further transferred on ½ MS media devoid of phytoharmones, containing activated charcoal (5%). After 10-15 days, rooting was achieved. In vitro regenerated shoots (5-6 cm long) with well-developed roots were transferred to pots containing vermiculite and soil (1:3) and maintained at high humidity.
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How to cite this article:
Manisha Sharma. Effect of different plant hormones on in vitro plant regeneration via somatic embryogenesis in Cajanus cajan L. millsp.. International Journal of Botany Studies, Volume 7, Issue 1, 2022, Pages 349-352
International Journal of Botany Studies International Journal of Botany Studies