Green alga Gayralia oxysperma sampled from three estuaries in central west cost of India i.e., Ratnagiri (RA), Achara (AC) and Terekhol (TE). In addition lipid peroxidation and hydrogen peroxide were analysed as oxidative stress markers, and activities of antioxidants i.e. enzymatic CAT, GST and non-enzymatic GSH and ASA were investigated for the understand their pollution levels and environmental condition. We observed a significance increase in lipid peroxidation, hydrogen peroxide and antioxidant enzymes of the RA site, suggesting that the seaweed at RA site are maximum as compared to remaining both the sites AC and TE. However, non- enzymatic ASA and GSH antioxidant was also observed high level in the RA site indicate (GSH and ASA) deu to the protection against stress metals. Study shows that changes in antioxidants at different sites in the G. oxysperma.