The objective of this piece of work was to assess phytotoxicity and effects on biochemical contents of Solanum lycopersicum seedlings cultivated under different concentration levels of tannery effluent (0, 10, 25, 50, 75 and 100 percent) and chromium (2.5, 5, 10, 25 and 50 mg/l). Chromium is among the most hazardous heavy metals found in tannery wastewater. As a result, the various chromium concentrations adopted in the current investigation were analyzed. When compared to control and other effluent treatments, the lower concentration 10 percent of tannery effluent improves tomato growth, photosynthetic pigments, and biochemical alterations such as amino acid, protein, sugar, and proline content. The noxious effects of chromium reduced the growth and other aspects gradually reduced in comparison to control.