There have been a some reports of the use of mushrooms for medicinal purposes, which may be due to lack of awareness. Mushrooms are woodland products that have been consumed for a long period as food, but very few have been used for medicinal purposes. In the proper hands, these magical organisms can be extremely beneficial in treating and preventing a wide range of disorders. The present study has been based on the Investigation of different Varieties of wild mushrooms, classified as edible and non-edible, containing some medicinal quality as per local societies of the Uttarakhand region. Uttarakhand is a northwestern Himalayan state which has a rich diversity of wild mushrooms due to good climatic conditions. There is a wide studying scope for mushrooms growing in this area. In the coming years, new novel drugs can be formulated and these novobiotics can act as a lifesaver for the people of the society. The present study is carried out in the monsoon season during the month of August 2019 to January 2020. A total of 18 macrofungi samples were collected, identified, which belong to 16 families from different geographical locations of Himalayan districts of the Uttarakhand region viz. Tehri, Rudraprayag Uttarkashi, Dehradun, Chamoli, and Pithoragarh region. The highest number of macrofungi was collected by Russulaceae, Polyporaceae, Amanitaceae, Ganodermataceae, and Agaricaceae families.