This study is focused on P. erinaceus in vitro culture and aims to improve the knowledge needed to contribute to its production. Plus trees seeds and softwood cuttings were used and the germination, resumption of development and growth of the aforementioned materials were monitored. The monitoring was conducted on Murashige & Skoog agar medium (MS0) under normal circumstances and on MS0 supplemented by growth regulators, auxin (MS-IBA) or cytokinine (MS-BAP). In vitro culture coupled with seeds going through a prior disinfection is successful with very low contamination (0-6%) and maximum germination capacity (90%). The seedlings growth monitored over 12weeks on MS0 is regular and characterized by a rejuvenation phenomenon observed in the leaves. There is also an aerial development after transplanting single-node segments from these seedlings on the same medium. Finally, for in vitro cutting, a positive reactivity of Plus trees cuttings cultured was illustrated by aerial morphogenesis. Based on the experimentations, MS0 is already proved effective. However, this result is accentuated through better budding percentages for 0.1mg/l IBA+0.5mg/l BAP medium. Furthermore, it is on 0.5mg/l IBA medium that the morphogenesis was more important. Finally, on all mediums, there were no rooting but instead a callus formation at the cuttings base.