After exploring numerous pharmaceutical databases such as PubMed, Google Scholar, Scopus, etc., it was observed that no combined anti-inflammatory perspectives of U. gambier standardized extract (UGSE) with V. wallichii standardized extract (VWSE) in an in vitro method (human red blood cells method) have been reported yet. Therefore, it was planned to screen both the extracts together to explore the synergistic activity. Also, the goal of the current study was to investigate potential anti-inflammatory applications for ointment formulations containing both UGSE and VWSE. The pharmaceutical properties of the formulations, such as pH, the skin irritancy test, viscosity, appearance, extrudability, spreadability, washability, and swelling index, were evaluated. The number of therapy choices for therapy has favorably expanded as a result of this study. Future uses for the created polyherbal formulations, which also contain extracts from Uncaria gambier and Valeriana wallichii, are conceivable. This finding brought back the ideas of ethnopharmacology in connection to modern medicine and explained the uses of polyherbal formulations in conventional medicine.