The objective of the study was to evaluate the phytochemical compounds present in methanolic root extract of M. hortensis. Phytochemical screening was carried out using GC-MS instrument following the standard protocol. GC-MS studies revealed the presence of 37 compounds in root extract of M. hortensis. Among them highest peak area (47.99%) was obtained by Guanosine. This study identifies the presence of pharmacologically active compounds in the plant extract which can be constructive for the formulation of novel drugs.