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VOL. 7, ISSUE 2 (2022)
Secondary metabolites present in novel endolichenic fungi isolated from Dirinaria applanata
Authors
Jake B Canapim, Jennifer L Luyun
Abstract
The conduct of this study generally aimed to identify the secondary metabolites present in the endolichenic fungi isolated from Dirinaria Applanata. Specifically, it sought to identify the secondary metabolites present in the novel endolichenic fungi isolated from Dirinaria applanata through thin layer chromatography. The host lichen was collected from Luquilu, Cabagan, Isabela. The isolated endolichenic fungi were morphologically characterized. Mass production of endolichenic fungi was carried out to increase the amount of fungal crude extracts. Presence of secondary metabolites in the fungal crude extracts was determined through Thin-Layer Chromatography. These extracts were spotted on TLC plates through the use of DCM-methanol extracting solvent. Spots were observed under a UV light at the wavelength of 254/356 nm. The TLC plates were sprayed with several spray reagents. In reference to Dragendorff’s reagent, brown orange spots indicate presence of alkaloids; triterpenes and sterols was determined through the presence of blue-violet spots after Vanillin-sulfuric acid reagent was sprayed on the TLC plate. Using ferricyanide-ferric chloride reagent, blue spots indicate presence of phenols, tannins and flavonoids. Also, brown spots produced after sprayingα-Naphthol-sulfuric shows positive results for glycosides. Lastly, presence of indoles can be detected using Van-Urk Salkowski test wherein positive result is indicated by blue-violet spots. Furthermore, the TLC plates were sprayed with several spray reagents and the visual appearance became the basis in identifying the compounds present. The isolated endolichenic fungi showed positive results to secondary metabolites including phenols, tannins, flavonoids, alkaloids, glycosides, indoles, triterpenes, and sterols.
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Pages:190-192
How to cite this article:
Jake B Canapim, Jennifer L Luyun "Secondary metabolites present in novel endolichenic fungi isolated from <em>Dirinaria applanata</em> ". International Journal of Botany Studies, Vol 7, Issue 2, 2022, Pages 190-192
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