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VOL. 7, ISSUE 2 (2022)
Isolation and comparative analysis of NBS-LRR disease resistance gene analogs in medicinal plant Drimia wightii.L
Authors
Hanumantha Rao HM, L Rajannna, S Prabhakara
Abstract
The NBS-LRR Type R- gene diversity is well studied in crop plants but there are very little data available in non-domesticated wild medicinal plants. This study explored the diversity of NBS-LRR Type R genes in the wild medicinal plant Drimia wightii. L and compared with characterized NBS Type R genes to understand the organization of R genes in wild plant genome. The NBS encoding sequences were isolated using degenerate PCR and TA cloning methodology. Sequence homology was conducted by using the NCBI-BLASTx tool; polymorphism was analyzed by DnaSP version 6 software. MEGAx software and Translate tool – ExPASy was used for obtaining protein sequences and motif analysis, protein alignment was edited and represented using JALVIEW. Further, DNA Multiple sequence alignment of NBS type RGAs were constructed by using the T-COFFEE tool and represented by BOXSHADE program. The methodology followed in this study helped to isolate seven NBS encoding sequences from D. wightii. L and nucleotide sequence was submitted to GenBank for unique accession numbers. The multiple sequence analysis showed only a few point mutations contributed to diversity. In addition, results disclosed the presence of parsimony information site containing sequences. The comparative analysis revealed that last residues of Kinase-2 motif are conserved and consists amino acid Tryptophan (W) which confirmed that all D.wightii. L sequence isolated is Non-TIR-NBS-LRR Type (CNLs). The results of the present study revealed that universal degenerate primers designed for highly conserved regions of the NBS domain (LM638, LM637) are efficient to amplify the NBS encoding genes in the wild medicinal plant Drimia wightii. This study further confirms the absence of TNLs in monocots genomes which were confirmed previously in cereal crop plant genomes. The importance of parsimony information sites in diversity analysis and identifying common ancestors was highlighted.
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Pages:168-178
How to cite this article:
Hanumantha Rao HM, L Rajannna, S Prabhakara "Isolation and comparative analysis of NBS-LRR disease resistance gene analogs in medicinal plant Drimia wightii.L". International Journal of Botany Studies, Vol 7, Issue 2, 2022, Pages 168-178
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