ARCHIVES
VOL. 7, ISSUE 2 (2022)
In vitro, combined anti-diabetic potential of S. oblonga linn. and E. littorale blume. extract
Authors
Sanjiv Duggal, Sanjeev Mittal
Abstract
The objective of the study was to evaluate the combined in-vitro inhibitory potential of Indian medicinal aqueous plants extract of S. oblonga Linn. stem and E. littorale Blume. aerial part using α-amylase and α-glucosidase enzymes. The both plants parts were extracted with water at 50°C for 30 minutes. The extracted material was concentrated in water bath and allows drying naturally. Different concentrations (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) of both extracts individually and in combination (1:1 ratio) were subjected to α-amylase and α-glucosidase inhibitory assay. The standard drug was Acarbose with which comparison was made. The absorbance was measured at 540 and 405 nm using a spectrophotometer. The percentage of α-amylase and α-glucosidase inhibitory activity and IC50 values of individual extract and the combined extracts were calculated. The results of the present study indicate aqueous extract of both the plants exhibited potential against diabetes mellitus in a dose dependent manner. Aqueous extract of E. littorale showed more inhibitory potential against α-glucosidase than α-amylase enzyme with IC50 values 0.31±2.12 mg/ml and 0.40±1.09 mg/ml respectively. However, the aqueous extract of S. oblonga showed appreciable α-amylase and α- glucosidase inhibitory activity with an IC50 values 0.42±2.01 mg/ml and 0.46±3.17 mg/ml, respectively, where as the acarbose showed IC50value of 0.17±1.21 mg/ml and 0.19±1.75 mg/ml against α-amylase and α- glucosidase enzyme respectively. The combined effect of the extract at (1:1 ratio) showed potential with IC50 value of 0.28±2.11 mg/ml and 0.21±1.14 mg/ml against α-amylase and α- glucosidase enzyme respectively. The present study results indicate that, both the Indian medicinal plants have potential to decrease the postprandial blood glucose level, which may due to phytochemical such as triterpenoids, flavonoids and phenolics active constituents in the extracts. Hence, this study supports the Ayurvedic concept of plants, especially in the management of type 2 diabetes. The efficacies of the combined extract indicate the stronger synergistic effect, when compared with individual inhibitory activity. This showed a beneficial aspect of a combination of herbal medicine formulation to reduce the postprandial hyperglycemia.
Download
Pages:151-155
How to cite this article:
Sanjiv Duggal, Sanjeev Mittal "<em>In vitro,</em> combined anti-diabetic potential of <em>S. oblonga</em> linn.<em> </em>and <em>E. littorale </em>blume<em>. </em>extract ". International Journal of Botany Studies, Vol 7, Issue 2, 2022, Pages 151-155
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.