Fusarium oxysporum f.sp.cumini causes cumin wilt disease all over the world. Chemical fungicides have significant environmental impacts and are hazardous to non-target organisms. Plant metabolites and plant-based pesticides appear to be among the best options since they are known to be less harmful to the environment and offer less risk to consumers than synthetic pesticides. Plant extracts of Vitex negundo were employed in an in vitro antifungal test against Fusarium oxysporum f. sp. cumini to create eco-friendly management. To extract the chosen plant, several solvent systems were utilised, including methanol, acetone, aqueous methanol, aqueous acetone, dichloromethanol:methanol, and water.The poison food technique was used to evaluate the antifungal activity of extracts at various concentrations (4 %, 6 %, 8 %, 10%, 12%, and 14 %) on FOC mycelial development and obtained a favourable result in the form of %inhibition. HPTLC fingerprinting was performed for some important phytochemicals like flavonoids, alkaloid and phenolic compound. This study suggests that botanical extracts could be a viable option for developing effective plant-based fungicides for the treatment of Fusarium oxysporum f. sp. cumini in organic farming.