Rhinacanthus nasutus (L.) Kurz.is a herbaceous shrub of the acanthaceae family. It is widely distributed in some regions of the subcontinent of India, China, and Southeast Asia. Rhinacanthus nasutus plant is widely used in folk medicine preparations, and recent research has begun to analyze its chemical composition and pharmacological properties. The purpose of this study was to identify the phytocomponents present in the chloroform extract of the Rhinacanthus nasutus leaves using GC-MS analysis. As a result of the GC-MS analysis, 18 compounds were identified. GC-MS analysis of the bioactive fraction revealed the highest peaks in the chromatogram. Chloroform extract of the Rhinacanthus nasutus (L.) Kurz. leaves contain a wide range of biologically important compounds. Therefore, our research's primary goal is to isolate these bioactive compounds and utilize them as therapeutic targets.