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VOL. 7, ISSUE 2 (2022)
in vitro propagation of Brassica nigra L. by using seeds
Authors
Swapna C, Accamma N P, Sharanya T, Ramesh B S, Jayarama Reddy
Abstract
The major outcome of plant tissue culture is the regeneration ability of plants invitrowhich is based on the principle of totipotency. The growth ability of plants is established by using organic media components which is supplemented with hormones and vitamins as growth regulators of varied concentrations. The work which mainly talks on the study of different concentrations of plant growth regulators used for the induction of callus formation and the proliferation of roots and shoots in different media compositions. In Brassica nigraL. the direct development of shoots and roots without formation of callus was seen, which is due to the usage of seeds. The seeds were cultured in MS medium with different concentrations and combination of Hormones.The media prepared wasMS1, MS2 and MS3 with different concentrations of growth regulators like auxin (NAA) and (BAP) which differed in three series of media. The Auxin and Cytokinin concentrations in MS2 media was kept more which was 5mg/L when compared to MS1 and MS3, so the growth rate in MS2 was more with the interval of time, so in the study it represented Murashige and Skoog is one of the most preferable media for the growth of mustard plant In vitro. The results obtained according to the time interval is presented in this paper.
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Pages:91-94
How to cite this article:
Swapna C, Accamma N P, Sharanya T, Ramesh B S, Jayarama Reddy "<em>in vitro</em> propagation of <em>Brassica nigra</em> L. by using seeds ". International Journal of Botany Studies, Vol 7, Issue 2, 2022, Pages 91-94
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