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VOL. 7, ISSUE 2 (2022)
Rapid growth of Trigonella foenum–graecum by In-vitro propagation methods
Authors
Thirtha M Shetty, Triveni, Tejashwini B N, Jayarama Reddy
Abstract
Fenugreek (Trigonellafoenum – graecum L.) is an annual herb and are widely cultivated. It is used as important vegetable, spice and mainly as medicinal plant that are used in some disease therapy (Moradi koret al. 2013).Fenugreek seeds contain a good amount of fiber and minerals, including iron and magnesium. The study Presents an efficient shoot generation protocolfrom within 8-10 days by using the fenugreek seeds for inoculation which is then cultured on Murashige and Skoog (MS) media with varied concentrations of IAA, BAP. The seeds are surface sterilized and inoculated into cultured medium with different concentrations of growth regulators. Presence of auxins in to the culture medium shows the positive increase in shoot length (Aasimet al.2010). The MS medium supplemented with hormone 6- Benzyl adenine at the concentration of required pH. In this study, the fenugreek seed shows the shoot initiation with the callus formation. There are few research papers shows that even the callus induction with different cytokinin and other different concentrations in hormones. This paper also shows the shoot generation and then callus formation after 20days with optimal concentrations of the hormones (IAA and BAP).
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Pages:68-71
How to cite this article:
Thirtha M Shetty, Triveni, Tejashwini B N, Jayarama Reddy "Rapid growth of <em>Trigonella foenum–graecum </em>by <em>In-vitro</em> propagation methods ". International Journal of Botany Studies, Vol 7, Issue 2, 2022, Pages 68-71
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