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VOL. 7, ISSUE 2 (2022)
Assessing the growth of Eleusine coracana seeds in artificial medium under In-vitro conditions
Authors
Neha Pal, Sharada Prajna, Ramesh B S, Jayarama Reddy
Abstract
An efficient reproducible plant regeneration of seed derived culture has been developed, a complete regeneration system has been developed using mature seeds as explants. The seeds of ragi were germinated and were successfully initiated in Murashige and Skoog’s media. Mesocotyl and leaf base tissue derived which gave shoot buds. The callus induction was seen on media with hormones mainly the MS media was supplemented with 9gm/lt agar along with hormones like auxin (IAA), cytokinin (BAP) both with 0.005 gm. /lt, sucrose 30gm/lt was added as the sole carbon source. Callus was successfully initiated in Vacin and Went media. MS Media showed seed germination and later gave out root and shoot, attributing that, MS media promoted direct organogenesis, whereas Vacin and Went media promoted indirect organogenesis by callus formation.
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Pages:123-126
How to cite this article:
Neha Pal, Sharada Prajna, Ramesh B S, Jayarama Reddy "Assessing the growth of <em>Eleusine coracana</em> seeds in artificial medium under <em>In-vitro</em> conditions ". International Journal of Botany Studies, Vol 7, Issue 2, 2022, Pages 123-126
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