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VOL. 7, ISSUE 2 (2022)
Anti-microbial activities of green synthesized Tagetes erecta L. leaf broth
Authors
K Dinesh Singh, Th Tilakraj Singh, M Suresh Singh, O Dwijamani Singh, P Devjani
Abstract
A simple, rapid, low cost and eco-friendly green synthesis of silver nanoparticles using the leaf broth of Tagetes erecta L. as a reducing agent has been investigated. The resulting silver nanoparticles were characterized using UV-Vis spectrophotometer, X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM) and Fourier Transformations Infra Red (FT1R) spectroscopic analysis. The identification of the crystallinity of silver nanoparticles was characterized using an XPert Pro X-ray diffractometer and the morphology of the synthesized silver nanoparticles by the Scanning Electron Microscope (FEI Quanta 250). XRD and SEM studies confirmed the formation of metallic silver nanoparticles with average crystallite size of 15.4 nm. The FTIR spectrum analysis of the synthesized silver nanoparticles reveals that biomolecules with carbonyl, hydroxyl and amine functional groups have the potential for metal ion reduction and for capping the newly formed nanoparticles. The antimicrobial activity was assayed by agar well diffusion method using 20 ml each of sterile Nutrient Agar (NA) (Hi-Media) and Potato-Dextrose Agar (PDA) (Hi- Media) for testing the bacterial and filamentous fungal activity respectively. Amphotericin-B (Hi-Media) for fungi and Ciprofloxacin (Hi- Media) for bacteria were used as control. The resulting silver nanoparticles exhibited moderate antimicrobial activities against the tested bacterial pathogens but not in fungal pathogens.
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Pages:5-9
How to cite this article:
K Dinesh Singh, Th Tilakraj Singh, M Suresh Singh, O Dwijamani Singh, P Devjani "Anti-microbial activities of green synthesized <em>Tagetes erecta </em>L<em>.</em> leaf broth ". International Journal of Botany Studies, Vol 7, Issue 2, 2022, Pages 5-9
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