Plants have the ability to synthesize a wide variety of secondary metabolites having a capacity to inhibit the pathogens growth. The present study appraise the antibacterial and antifungal activity of ferulic acid- an isolated phenolic compound from pineapple peel against four different bacteria namely Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus and Bacillus cereus and five pathogenic fungi such as Alternaria alternata, Fusarium oxysporum, Aspergillus niger, Colletotrichum gloeosporioides and Candida albicans using agar well diffusion method. Different concentrations of ferulic acid ranging from 250 μg to 1000 μg were tested against the selected plant pathogenic fungi whereas ferulic acid 500µg and 1000µg were evaluated against gram positive and gram negative bacteria and the inhibition zone were compared against standard drug Ciprofloxacin (2µg) and Clotrimazole (100µg) respectively as positive control. Ferulic acid showed maximum potentiality against Bacillus cereus - a gram positive bacteria and with the most common fungal plant pathogens namely Colletotrichum gloeosporioides. Results from the study showed that the ferulic acid isolated from the pineapple waste has significant antimicrobial activity against harmful pathogenic microorganisms. This biologically active phenolic compound can be further studied to develop new antimicrobial drug.